The vast majority of Smart Tube PROT1 users over the past 10 years have used Sodium Heparin as the anticoagulant and all of the initial validation was with Sodium Heparin due to the fact that EDTA and Citrate chelate extracellular calcium and thereby have an impact (undesirable in most cases) on cell signaling.
In comparison, Sodium Heparin is thought to be a much better choice when intracellular signaling is a key element of the study.
As a result, there has been little use of EDTA or Sodium Citrate with the PROT1 reagent system so performance of these anticoagulants with the PROT1 reagent system is unknown. There has not been extensive use of the system with Lithium Heparin as the anticoagulant, but Lithium Heparin, just like Sodium Heparin, is unlikely to interact with the PROT1 chemistry.
To conclude, Sodium Heparin is the most well-tested choice. Lithium Heparin is likely to perform similarly, but has not been extensively used with the system. EDTA and Sodium Citrate will impact cell signaling and are not recommended.
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