When banking samples with Smart Tube products including Smart Tubes, PROT1, or the Stable-Lyse Stable-Store reagent system, Sodium Heparin is the recommended anticoagulant.
The anticoagulants EDTA and Citrate prevent coagulation by chelating extracellular calcium and thereby have an impact (undesirable in most cases) on intracellular signaling. In comparison, Sodium Heparin is thought to be a much better choice when intracellular signaling is a key element of the study. Analysis of intracellular signaling is a component of much of the work utilizing Smart Tube products and as a result there has been less use of EDTA or Sodium Citrate with Smart Tube products.
Check out our Publications Page to see examples of clinical trials and studies that banked their samples with Smart Tube products and examined intracellular in addition to cell surface phenotype.
Tags: anticoagulant, anti-coagulant, sodium heparin, heparin, EDTA, Sodium Citrate, Citrate, chelating, calcium, calcium signaling, intracellular signaling, problems detecting intracellular signaling
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