Since the reaction of the PROT1 with the blood sample can be approximated with the Arrhenius equation, the reaction rate will be cut in half for every 10 degrees Celsius drop in temperature. Because of this, to ensure proper preservation of the blood sample, both the blood sample and the PROT should both be at room temperature before initiating the reaction.
If the overall reaction temperature is too low then the sample will not be adequately stabilized before being transferred to the freezer.
The following product may be helpful:
Sticker to make sure the PROT1 is at room temperature before using (should not be stored below room temperature but this would be visual confirmation that it is at room temperature):
https://www.zebra.com/us/en/products/environmental-sensors/ready-to-use-indicators/limitmarker-reversible.html
Use the same type of sticker on the Vacutainer to make sure the blood is at room temperature before adding to the PROT1
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