Use of cleaved PARP to identify dying cells by CyTOF or by conventional flow cytometry when they can't be gated out by FSC SSC alone:
Epitope: D214
Clone: F21-852 (BD Biosciences is one supplier of this clone)
Publications that used this antibody with Smart Tube stabilized samples include:
Single-cell mass cytometry adapted to measurements of the cell cycle
Cytometry Part A
Gregory K. Behbehani, Sean C. Bendall, Matthew R. Clutter, Wendy J. Fantl, Garry P. Nolan
and:
Mass cytometric functional profiling of acute myeloid leukemia defines cell cycle and immunophenotypic properties that correlate with known responses to therapy
Cancer Discovery, 2015
Behbehani et al
and methods paper:
Cell Cycle Analysis by Mass Cytometry.
Methods Mol Biol. 2018;1686:105-124. doi: 10.1007/978-1-4939-7371-2_8
Gregory K. Behbehani
Use of cleaved PARP to identify dying cells
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