Ensure that the ratio of PROT1 to the blood sample is 1.4 to 1 and that the PROT1 is at room temperature when it is used. As long as the ratio remains 1.4 to 1 this can be scaled up to larger volumes of blood:
- 14 milliliters of PROT1 for 10 milliliters of whole blood (ie. when investigating extremely rare populations such as cancer stem cells)
or scaled down to smaller volumes of whole blood:
- 140 microliters of PROT1 for 100 microliters of whole blood (ie. when banking samples from pediatric patients)
Protocol for banking whole blood samples with PROT1:
https://www.smarttubeinc.com/protocols/prot1/ST_BANK_STP1TF-120101B.pdf
Protocol for Thawing and Lysing erythrocytes in whole blood samples banked with PROT1:
https://www.smarttubeinc.com/protocols/prot1/ST_THAW_STTLTF-130528B.pdf
You can purchase PROT1 from our Online Store here : https://order.smarttubeinc.com/collections/proteomic-stabilizer-prot1
Is it OK to store in Falcon 50 mL polypropylene tubes?
Yes, use labware made of polypropylene or polyethylene such as a cryovial or conical tube. If you are going to ship PROT1 you should probably look into the Nalgene "PASSPORT IP2" series like part number 2099-0004 https://www.usplastic.com/catalog/item.aspx?itemid=135938
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